Composite

Part:BBa_K1897029

Designed by: Keshiniy Madivannan   Group: iGEM16_NUS_Singapore   (2016-10-11)
Revision as of 17:45, 18 October 2016 by Phuongnguyen (Talk | contribs)


p62-33-sfGFP-Terminator

The construct contains a wild-type lldPRD operon promoter from E. coli (Part:BBa_K822000) with a weaker RBS flanked by non-coding sequences (Part:BBa_K1897031), followed by a coding sequence of sfGFP (Part:BBa_K1897033) and a lambda t0 terminator (Part:BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 589
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None