Device

Part:BBa_K1886002:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)
Revision as of 21:03, 19 October 2016 by Lejian (Talk | contribs) (→‎References)

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AHL,DPD double-cycled oscillation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
    Illegal BamHI site found at 4352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3256
    Illegal AgeI site found at 4450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1970
    Illegal BsaI.rc site found at 3073
    Illegal BsaI.rc site found at 4114


Design Notes

LAA tag has the highest degradation efficiecy in 37℃. Pay attention to the environmental temperature while using it.It can only be used in Gram-negative bacteria.



Source

From E coli genome, the subparts of this part is from iGEM Distribution kits.

References

Danino T, Mondragón-Palomino O, Tsimring L, et al. A synchronized quorum of genetic clocks[J]. Nature, 2010, 463(7279): 326-330.