Part:BBa_K1856000

bacA-citrine-T7 terminator

bacA promoter assembled to citrine and T7 terminator

Usage and Biology

bacA is an inducible promoter native to rhizobium species (notably R. tropici and S. meliloti) that is induced by flavonoids. This construct has been successfully cloned into E. coli using the broad-host range vector pKT230, a RSF1010 derived plasmid. Leaky expression of citrine was observed.

The construct has also been cloned into S. meliloti.

Characterization

Measured strengths

Leaky expression of BacA was observed See part J61002 for details on their use.

Obtaining the Anderson promoter collection

The sequences of construct can be found via the table below. The physical DNA can be obtained from:

Via request: The Yale iGEM team has the promoter cloned

Via the Registry distribution: The promoters are included in the Registry distribution. All parts except BBa_J23119 are present in plasmid BBa_J61002. This places the RFP downstream of the promoter. Part BBa_J23119 is present in pSB1A2.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 920
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 920
  Illegal NotI site found at 945
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 920
  Illegal BamHI site found at 914
  Illegal XhoI site found at 954
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 920
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 920
  
• 1000
  COMPATIBLE WITH RFC[1000]