Part:BBa_K1841001
nisI
Research from Torsten et al. revealed that the highest level of acquired nisin tolerance was achieved after coordinated expression of all four nisin immunity genes, containing nisI, nisF, nisE, and nisG. Functional analyses provided evidence that NisI acts as a nisin-sequestering protein that expels nisin molecules from the cytoplasmic membrane into the environment.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2019-SZPT-China
1.Construction of pMG36e-nisl vector
Since the biological chassis is ultimately to be valued in the intestine, a food grade resistant expression vector is required. We replaced the erythromycin resistance gene of the original plasmid PMG36e with the nisin resistance gene nisl，which encode an antibacterial peptide for food preservation. Fig13 is the result of recombinant pMG36e-Nisl verification. The restriction and PCR result show that nisl with size 862bp was inserted in pMG36e successfully.
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