Coding

Part:BBa_K1824018

Designed by: Zhe Yang   Group: iGEM15_XJTLU-CHINA   (2015-09-10)
Revision as of 15:30, 17 September 2015 by Wenbo (Talk | contribs)

aeBlue Blue chormoprotein with lva tag

AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012. We add lva tag to before stop codon to increase the degradation of the Aeblue chromoprotein, which is necessary in our earth map for color changing.

Characterization

XJTLU-CHINA tested accumulation status of blue chromoprotein with two different fast digestion tag,lva and aav tag, under the regulation of T7 promter-lac operator in E.coil strain BL21 (DE 3).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None