Composite

Part:BBa_K180019

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-19)

Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]

Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.

mBanana and LuxI are generated under tac pR regulation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1825
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1825
    Illegal NgoMIV site found at 2071
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2014


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
emission553 nm
emit553
excitation540 nm
excite540
lum
n/aScissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]
proteinmBanana