Composite

Part:BBa_K180003

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-16)

PTAC testing plasmid (GFP) - basic

As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.

The lacIq sequence preceding the tac promoter ensures that tac pR is in the 'OFF' state naturally. Green fluorescent protein is produced if IPTG successfully induces the promoter, demonstrating the regulatory effect of Lac upon the promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2097


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
emission511 nm
emit511
excitation501 nm
excite501
lum
n/atac pR testing plasmid (GFP) - basic
proteinGFPmut3b
tagNone