Coding

Part:BBa_K1728021

Designed by: Wan-Yun Chiu, Ming-Fei Hsieh   Group: iGEM15_CGU_Taiwan   (2015-09-17)

Chemokine receptor 1 (CXCR1)

Chemokine receptor 1 (CXCR1) CXCR1 (also named with IL8RA and CD181) has high affinity in binding with IL8. CXCR1 belongs to the GPCR rhodopsin-like family and has seven-transmembrane domains, with the N-terminus exposed to the extracellular space and the C-terminus exposed to cytoplasm. Different from ordinary GPCR ligand binding sites, in which of CXCR1 locate on N-terminal and extracellular loop 3 of CXCR1. To improve heterologous expression of CXCR1 in yeast, we inserted two different signal peptide sequences upstream of the CXCR1 sequence. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 786
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 211
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 975


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None