Coding

Part:BBa_K1680022:Design

Designed by: Nicolai von Kuegelgen   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 01:35, 26 September 2015 by KatharinaS (Talk | contribs) (→‎References)


Dronpa caged Cre with NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 298
    Illegal BglII site found at 2050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1163


Design Notes

3A Assembly using RFC25


Source

gBlock designed by Team Tuebingen

References

Zhou, X. X., Chung, H. K., Lam, A. J., & Lin, M. Z. (2012). Optical control of protein activity by fluorescent protein domains. Science, 338(6108), 810-814. doi: 10.1126/science.1226854