Coding

Part:BBa_K1680021:Design

Designed by: Nicolai von Kuegelgen   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 01:06, 26 September 2015 by KatharinaS (Talk | contribs) (→‎References)


Dronpa caged Cre with NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 298
    Illegal BglII site found at 2032
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1154
    Illegal SapI site found at 704
    Illegal SapI site found at 1751


Design Notes

3A Assembly using RFC25


Source

gBlock design by Team Tuebingen

References

Zhou, X. X., Chung, H. K., Lam, A. J., & Lin, M. Z. (2012). Optical control of protein activity by fluorescent protein domains. Science, 338(6108), 810-814. doi: 10.1126/science.1226854