Composite

Part:BBa_K1658005

Designed by: Şeniz Yüksel   Group: iGEM15_METU_HS_ANKARA_   (2015-09-18)

Limonane + GFP regulator

After ligation of I742111 and E0240, we have complete the circuit with constitutive promoter(J23116) and the difference between I742111+E0240 and J231116+I742111+E0240 can be seen by analyzing three lanes after ladder.

Figure1:As characterization of previously submitted parts, we have ligated RBS+Limonene synthase gene coding part and GFP reporter gene and obtain lane at approximatly 2500 basepairs after double digestion

Design Notes

RBS+Limonene Synthase ,tranitional unit, was ligated with the Reporter biobrick includes RBS(B0032), GFP coding region(E0040) and double terminator(B0010-B0012). The aim was to obtain flourescence under spectrophotometry to measure the production of limonene synthase after ligating with constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2358
    Illegal SapI.rc site found at 116


[edit]
Categories
//awards/part_collection/2015
Parameters
None