Part:BBa_K1631002

Translational unit of Colicin-E3

Colicin-E3 cleave 16SrRNA at a specific site. This activity is inhibited by immunity protein for Colicin-E3(BBa_1631004).
When lysis protein for Colicin-E3(BBa_1631003) is co-expressed, Colicin-E3 is released to environment and kill other strain.

Colicins are type of bacteriocin produced by Escherichia coli.

Usage and Biology

　Colicins are a cytotoxins which are released to environment and kill other related strains.

Release of colicin involves one protein; Colicin Lysis Protein. Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] After Colicin released, they diffuse through the medium and bind to the receptor on the target cell membrane. Then, they are imported to the cytoplasm or cytoplasmic membrane of target cell by Tol-system or Ton-system. (If you want to know the mechanism of the Colicin import, see [1])
Colicin producing cells also express Colicin Immunity Protein in order to protect themselves from cytotoxity of colicin.
Colicins have variety cytotoxity such as DNase activity, RNase activity or Pore forming across inner membrane.

Reference
[1]Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubes, R., Postle, K., ... & Cavard, D. (2007). Colicin biology. Microbiology and Molecular Biology Reviews, 71(1), 158-229.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1526
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 489
  Illegal AgeI site found at 241
  Illegal AgeI site found at 1507
  Illegal AgeI site found at 1612
  
• 1000
  COMPATIBLE WITH RFC[1000]