Device
anti X

Part:BBa_K1585350:Design

Designed by: Laura Helleckes, Michael Osthege, Sarah Lubjuhn   Group: iGEM15_Aachen   (2015-09-05)
Revision as of 23:54, 5 September 2015 by Mosthege (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


glgX targeting plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 167
    Illegal XbaI site found at 173
    Illegal SpeI site found at 58
    Illegal PstI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 167
    Illegal NheI site found at 35
    Illegal SpeI site found at 58
    Illegal PstI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 167
    Illegal BglII site found at 1015
    Illegal BamHI site found at 23
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 167
    Illegal XbaI site found at 173
    Illegal SpeI site found at 58
    Illegal PstI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 167
    Illegal XbaI site found at 173
    Illegal SpeI site found at 58
    Illegal PstI site found at 185
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not designed to be BioBrick-compatible, but it can be used AS IS to knock out glgX in E. coli.

It has to be used together with pCas (https://www.addgene.org/62225/)


Source

The part consists of genomic repair templates with a gene-edit and a synthetic sgRNA sequence.

References

http://www.ncbi.nlm.nih.gov/pubmed/25636838