Composite

Part:BBa_K1520013

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter

Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.


K1520010.jpg

From left to right, Marker(DL10000), Prlux, Prlux-rbs-rfp, Prlux-rbs-rfp-Ter, Prlux-rbs-rfp-rbs-luxI, Prlux-rbs-rfp-rbs-luxI-Ter, Prlux-rbs-rfp-rbs-luxR-Ter, Prlux-rbs-rfp-luxR, Prlux-rbs-rfp-luxI-rbs-luR, Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1458
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
Parameters
controlluxR, HSL
directionForward
ec_numnone
functionMixed
keggnone
ligandsHSL
negative_regulators
o_h
o_l
positive_regulators1
proteinLuxR
swissproP12746
tagNone
uniprotP48445