Composite
Part:BBa_K1520009
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Plac-rbs-luxI-Ter
Plac-rbs-luxI-Ter.
It produces luxI when there is less lacI or most of lacI is occupied by IPTG.
From left to right, Marker（DL2000）, Plac, Plac-rbs-luxI, Plac-rbs-luxI-T. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis/ahl
//function/cellsignalling
//function/cellsignalling
| biology | |
| control | IPTG, lacI |
| direction | Forward |
| ec_num | none |
| kegg | none |
| negative_regulators | 1 |
| o_h | |
| o_l | |
| positive_regulators | |
| protein | luxI-LVA |
| swisspro | P12747 |
| tag | LVA |
| uniprot | P48445 |