Conjugation

Part:BBa_K1510624

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-08)


Truncated lysostaphin coding sequence plus strong promoter and yebf


we use truncated lysostaphin coding sequence(designed by HIT-Harbin 2012 igem team) to reach our goal deplete biofilm formed by streptococcus mutans. YebF,a signal protein, would be secreted outside E.coli to make protein works more accurately.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal NheI site found at 73
    Illegal NheI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None