Conjugation

Part:BBa_K1510603

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-08)


Dispersin generator ,with constitutive promoter plus yebf

The Dispersin gene codes for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram negative bacteria.With strong constitutive promoter,together with ribosomal binding site and signal sequence yebF, make dispersin work more accurately.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal NheI site found at 73
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 202
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1267
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None