Coding

Part:BBa_K1499501:Design

Designed by: Jovita Byemerwa   Group: iGEM14_StanfordBrownSpelman   (2014-09-29)
Revision as of 18:51, 2 November 2014 by Raman2004 (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Endo-1,4-beta-glucanase (cellulase gene)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the gene is usually secreted by the original bacterium (Neisseria Sicca), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene.

Source

Genomic sequence of Neisseria Sicca SB

References