Coding

Part:BBa_K1499401:Experience

Designed by: Ian Hull   Group: iGEM14_StanfordBrownSpelman   (2014-10-09)
Revision as of 00:52, 25 October 2014 by Ianhull (Talk | contribs) (→‎Applications of BBa_K1499401)


To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.

Figure 1. C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.

Applications of BBa_K1499401

The protein encoded by this gene has shown potential chitin-binding activity. Pending assays for cellulose-binding activity and hydrophobicity, this protein could be used to waterproof chitin- and cellulose-based biomaterials – specifically in our case, fungal mycelium and bacterial cellulose.

User Reviews

UNIQ0953aeaf6c94630f-partinfo-00000000-QINU UNIQ0953aeaf6c94630f-partinfo-00000001-QINU