Part:BBa_K1499401:Experience

To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that PdomMRNAr1.2-08705.1 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.

Figure 1. C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.

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