Coding
Part:BBa_K1499400:Experience
Designed by: Ian Hull Group: iGEM14_StanfordBrownSpelman (2014-10-09)
To attempt to purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499400 (nicknamed C1) was not successfully purified with the chitin magnetic beads, suggesting that it may not have the chitin-binding activity predicted by PSI-BLAST.
Figure 1. C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. Although C1 did not show up on the gel, C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST. See the page for BBa_K1499401 for more information on C2.
Applications of BBa_K1499400
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UNIQebe2cc3e8b19c38b-partinfo-00000000-QINU UNIQebe2cc3e8b19c38b-partinfo-00000001-QINU