Coding

Part:BBa_K1499400:Experience

Designed by: Ian Hull   Group: iGEM14_StanfordBrownSpelman   (2014-10-09)
Revision as of 00:56, 25 October 2014 by Ianhull (Talk | contribs)

To attempt to purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499400 (nicknamed C1) was not successfully purified with the chitin magnetic beads, suggesting that it may not have the chitin-binding activity predicted by PSI-BLAST.

Figure 1. C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.

Applications of BBa_K1499400

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UNIQa33181797766b4a8-partinfo-00000000-QINU UNIQa33181797766b4a8-partinfo-00000001-QINU