Part:BBa_K1499400:Experience

To attempt to purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499400 (nicknamed C1) was not successfully purified with the chitin magnetic beads, suggesting that it may not have the chitin-binding activity predicted by PSI-BLAST. BBa_K1499401 (C2), however, did show chitin-binding activity.

Figure 1. C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.

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