Coding

Part:BBa_K1484101:Design

Designed by: Salil Bhate   Group: iGEM14_Cambridge-JIC   (2014-10-08)
Revision as of 11:54, 10 October 2014 by Salil (Talk | contribs)

GAL4-VP16 transcription activator CDS, compatible with PlantSyntax (RFC105)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 226
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 695
    Illegal SapI site found at 206


Design Notes

Restriction sites removed for RFC10. Sites added for compatibility with RFC105 (PlantSyntax).

Source

J. Haseloff

References