RBS
NKRF/FG

Part:BBa_K1442110

Designed by: Caroline de Cock   Group: iGEM14_Warwick   (2014-10-07)

NKRF IRES with forward GFP

Construction

This was the complete testing module used in the testing of the NKRF IRES, results can be seen on Part:BBa_K1442024. This uses the empty testing GFP sequence Part:BBa_K1442106, which has been codon optimised for human use using the IDT codon optimiser from the original gene derived from Aequorea victoria jellyfish gene Part:BBa_E0040.

This was measured in Huh 7.5 and HeLa cell with a tecan magellan machine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1289
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 375
    Illegal SapI site found at 734


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Categories
Parameters
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