RBS

Part:BBa_K1419003

Designed by: Harland Brandon   Group: iGEM14_Lethbridge   (2014-10-09)

Universal riboregulatable RBS

Universal RNA-IN RBS part

Usage and Biology

Allows for the riboregulation of translation of proteins expresses using this RBS. Based off of the Elowitz RBS (B0034)

Contains the S32 RNA-IN sequence from Mutalik et. al. (2012) spaced appropriately upstream from the RBS. Downstream of the RBS is a start codon and the RFC 25 suffix. When assembled with a protein coding part, there will be an addition of 6 amino acids to the N-terminus. This is necessary to allow for a single RNA-OUT molecule to regulate any protein expressed using this RBS. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None