Project

Part:BBa_K1314013

Designed by: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting   Group: iGEM14_Hong_Kong-CUHK   (2014-10-07)
Revision as of 18:30, 25 October 2014 by Lilyy (Talk | contribs)

nifK-XhoI-nifH promoter T7 expression system(strong)

homologous integration knock out nitrogenase gene with nifH promoter T7 expression system

Usage and Biology

Picture2.png


Fig: EcoRI PstI double digest check gel photo


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3281
    Illegal XhoI site found at 171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4023
    Illegal AgeI site found at 4135
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None