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Part:BBa_K1216000:Design

Designed by: Angela Aeschbach   Group: iGEM13_ETH_Zurich   (2013-08-29)
Revision as of 03:43, 5 October 2013 by Blockmoka (Talk | contribs) (→‎Design Notes)

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β-Glucuronidase (gusA) from Bacillis Subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No illegal restriction sites within the part

We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites and further cloning steps

Source

Bacillus subtilis.

References