Difference between revisions of "Part:BBa K1184000:Experience"
Enpederson (Talk | contribs) (→User Reviews) |
Enpederson (Talk | contribs) (→User Reviews) |
||
| Line 26: | Line 26: | ||
[[image:KillerRed_RFP_Phototoxicity.png|thumb|600px|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.]] | [[image:KillerRed_RFP_Phototoxicity.png|thumb|600px|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.]] | ||
<p>KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1.<br /> | <p>KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1.<br /> | ||
| − | RFP 66% ± 20% <br /> | + | RFP 66% ± 20% culture alive after photobleaching<br /> |
KillerRed: 51% ± 17% culture alive after photobleaching | KillerRed: 51% ± 17% culture alive after photobleaching | ||
</p> | </p> | ||
Revision as of 18:50, 14 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1184000
User Reviews
UNIQab9261a4ebb96bb5-partinfo-00000000-QINU
|
•••
Carnegie_Mellon 2013 |
 Figure 1: Phototoxicity of KillerRed and mRFP1 (BBa_E1010) on E. coli XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours. KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1. XL10 Ultracompetent cells were transformed with BBa_K1184000 with BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.
|
