Coding

Part:BBa_K1184000:Design

Designed by: Eric Pederson   Group: iGEM13_Carnegie_Mellon   (2013-09-04)
Revision as of 20:20, 4 September 2013 by Enpederson (Talk | contribs) (→‎Design Notes)


KillerRed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151
    Illegal BsaI.rc site found at 442


Design Notes

This sequence is not codon optimized for E. coli. Sequence contains 14 rare proline codons (CCC) and a rare arginine codon (AGA).

KillerRed has catalytic residues E70 and S121. The chromophore forms from the backbone of Q67, Y68 and G69.1

Source

Engineered from anm2CP. Provided by the Bruchez lab at Carnegie Mellon

References