Coding
Part:BBa_K1152012:Experience
Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis Group: iGEM13_Heidelberg (2013-09-20)
DelC 4'-Phosphopanthetheinyl-transferase
- CDS of sfp derived from Delftia acidovorans SPH-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 288
Design Notes
The delC CDS is derived from Delftia acidovorans SPH-1. [Johnston, 2013] We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.