Part:BBa_K1150031
uniCAS UV Light Switch Part II - Repressor
| CMV:COP1-Linker-KRAB-NLS:BGH | |
|---|---|
| Function | Repression domain of UVB light
induced gene expression control |
| Use in | Mammalians |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
The fusion protein COP1-KRAB is an interaction partner of dCas9-UVR8 and part of the UVB light controlled mechanism to repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-KRAB protein. This represses gene expression controlled by UVB light.
For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#repression repression] project page.
Biology and Usage
E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1. For this UVB light controled gene activation system you need a combination of these BioBricks: dCas9-UVR8, COP1-KRAB and RNaimer.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal BsaI.rc site found at 1876
Illegal SapI.rc site found at 962
Illegal SapI.rc site found at 1840
References
[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332
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