Part:BBa_K1150004
PhyB
| PhyB | |
|---|---|
| Function | Photoreceptor |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Organism | Arabidopsis thaliana |
| Source | AG Weber, Freiburg |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Phytochrome B is a photoreceptor which detects red and far-red light. In Arabidopsis thaliana, Phytochrome B predominantly controls seedling establishment. It is a protein with a molecular mass of 125 kDa that is predominantly located in the cytoplasm. Its structure can be roughly divided in two parts: The N-terminal regulatory and photosensory part and the C-terminal regulatory part. The N-terminal part is covalently bound to phytochromibilin. Phytochromibilin is a linear tetrapyrol that undergoes conformational changes upon radiation of either red- or far red-light when bound to the phytochrome moiety. This conformational change is responsible for the photosensory properties of PhyB. [1]
Illumination with red light (660 nm wavelength) leads to binding of Phytochrome Interaction Factor 6 (PIF6). The interaction can be abolished by illumination with far-red light (740 nm) wavelength.
Team Freiburg 2013 uses Phy B to induce gene activation or repression via light stimulus. Therefore Phy B can be fused to effector domains like VP16 or KRAB.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 490
Illegal BamHI site found at 572
Illegal XhoI site found at 523
Illegal XhoI site found at 542 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 739
References
[1] Lars-Oliver Essen, Jo Mailliet, and Jon Hughes 2008; Quail 2002
| None |