Part:BBa_K110016

A-Cell Promoter STE2 (backwards)

This promoter was isolated by taking 500bp upstream of the STE2 gene of S. cerevisiae, which encodes the α-factor pheromone receptor found on mating-type a cells. This promoter will be activated when a factor is expressed in an a haploid yeast cell. This is the same sequence as the STE2 promoter(BBa_K11009), but with suffix and prefix switched (right to left).

Usage and Biology

The receptor encoded by STE2 is a seven transmembrane-domain G protein-coupled receptor that binds to α-factor secreted by MATα cells to initiate the shmooing, cell cycle arrest, and the signaling response that leads to mating between haploid a and α cells.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]