Regulatory

Part:BBa_K110006

Designed by: James DiCarlo   Group: iGEM08_Johns_Hopkins   (2008-05-30)

Alpha-Cell Promoter MF(ALPHA)1

This promoter was isolated by taking 500bp upstream of the MFα1 gene of S. cerevisiae, which encodes the α-factor mating-type α cells. This promoter will be activated when a factor is expressed in an a haploid yeast cell. This is the same sequence as the MFα1 promoter(BBa_K11005), but with suffix and prefix switched (right to left).

Usage and Biology

The MFα1 gene encodes the Mating factor α pheromone. This pheromone is secreted into the surrounding media and it induces shmoo formation, and mating in MAT-a haploid yeast cells. This promoter should be strong if used in haploid α-cells S. cerevisiae cells


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
Parameters
negative_regulators
positive_regulators1