Part:BBa_K1092003

T7-RBS-Dioxygenase

This is a Biobrick of Catechol 1,2-Dioxygenase containing a T7 promoter and ribosomal binding site (RBS).

Catechol 1,2-dioxygenase (EC 1.13.11.1) from Pseudomonas putida KT 2400 is an oxidative enzyme which induces ortho-ring cleavage of catechol, a phenolic compound (Cao et al., 2008, Kim et al., 2006). Since catechol is the intermediate of various metabolisms related to aromatic compounds degradation, catechol 1,2-dioxygenase may be significant for the complete degradation of these compounds (Cao et al., 2008). Moreover, the previous study of Hadibarata et al. indicated that 1,2-dioxygenase may play a very important role in benzo[a]pyrene degradation (Hadibrata et al., 2012). Therefore, this enzymes was included for the proposed metabolic pathway of PAHs in the project. The gene sequence of this dioxygenase is readily available in GenBank with the reference code of 1045930.

Properties of Catechol 1,2-dioxygenase:

Optimal temperature: 25~30°C;

Optimal pH: 7.5~8 (Kim et al., 2006).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 176
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]