Generator

Part:BBa_K1072002

Designed by: Yichong wang, Ruichen, Xihao liao and Junjie zhang   Group: iGEM13_SCUT   (2013-09-01)

NADH dehydrogenase

BBa_K1072002 is the generator of NADH dehydrogenase which is an enzyme following chemical reaction:

NADH + H+ acceptor \rightleftharpoons NAD+ + reduced acceptor


We utilize the characteristic that H2O2 has absorbance at 240nm to complete the linear chart of standard H2O2 concentration as figure 1 by UV spectrophotometer.We choose part BBa_K546000 as the control for part BBa_K1072003 and get the concentration of H2O2 which is 7.21*10-2 mol/L.BBa_K1072003 is the composite of BBa_K1072002 and BBa_K546000.

Figure 1. the linear chart of standard H2O2 concentration



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/enzyme
//cds/transcriptionalregulator/activator
//cds/transcriptionalregulator/repressor
//function/cellsignalling
//function/regulation/transcriptional
Parameters
directionForward
proteinNADH dehydrogenase