Plasmid_Backbone

Part:BBa_K106698

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-29)


AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)

Acceptor for AarI AD parts, with 8X LexA operators directly upstream of the medium strong Cyc1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
    Illegal NotI site found at 4048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
    Illegal BamHI site found at 4031
    Illegal XhoI site found at 3965
    Illegal XhoI site found at 4950
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6244
    Illegal NgoMIV site found at 3133
    Illegal NgoMIV site found at 4561
    Illegal AgeI site found at 5857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1430
    Illegal SapI.rc site found at 408
    Illegal SapI.rc site found at 2512


[edit]
Categories
//chassis/eukaryote/yeast
//plasmidbackbone/operation
Parameters
None