Plasmid_Backbone

Part:BBa_K106697

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)

This is a variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t), with the addition of 8X LexA operator sites directly downstream of the Adht1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
    Illegal NotI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
    Illegal BamHI site found at 5
    Illegal XhoI site found at 1086
    Illegal XhoI site found at 6550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
    Illegal NgoMIV site found at 697
    Illegal AgeI site found at 1993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4930
    Illegal SapI.rc site found at 3908
    Illegal SapI.rc site found at 6012


[edit]
Categories
//chassis/eukaryote/yeast
//plasmidbackbone/operation
Parameters
None