Plasmid_Backbone

Part:BBa_K106693

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)

This is a special variant acceptor vector for AarI cloning. The "A" site used here, termed "A!" is an alternate end used when no amino-terminal fusions are tolerated. Please see the UCSF 2008 iGEM wiki for more information on AarI cloning.

This was designed specifically for use with Sir3 and amino-terminal domains of Sir3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal NotI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal BamHI site found at 5
    Illegal XhoI site found at 924
    Illegal XhoI site found at 7641
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 6360
    Illegal AgeI site found at 1831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4768
    Illegal BsaI site found at 7010
    Illegal SapI.rc site found at 3746
    Illegal SapI.rc site found at 5850


[edit]
Categories
//chassis/eukaryote/yeast
//plasmidbackbone/operation
Parameters
None