Generator

Part:BBa_K1060014

Designed by: Ingmar Claes, Sylvie Weckx, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-30)
Revision as of 09:52, 30 September 2013 by IngmarClaes (Talk | contribs) (space after komma)

Strong constitutive expression of EBF synthase from Artemisia annua

For the production of the alarmhormone, (E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a strong (constitutive) promoter and strong RBS (BBa_K608002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1367
    Illegal BamHI site found at 676
    Illegal XhoI site found at 638
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 248


[edit]
Categories
Parameters
None