Part:BBa_K1060009
Medium constitutive expression of EBF synthase from Artemisia annua
For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 177 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1368
Illegal BamHI site found at 677
Illegal XhoI site found at 639 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 249
Construct digestion
System testing
Our pilot experiment with aphid indicated that our brick performed the desired function, for the video of aphids experiment, please go to [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video].
In this video you will find our first behavioural experiment with aphids. An EBF-producing bacterium plate (E. coli BL21(DE3) on LB with 5mM Mg2+ and Chloramphenicol is placed on the left, a control plate on the right, aphids are placed on the leaf in the middle. The first results indicate a positive trend, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.) For more information on the experimental setup and a discussion of the results go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here].
For more infromation see the experience page or go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here].
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