Generator

Part:BBa_K1060009

Designed by: Ingmar Claes, Su Wang, Sylvie Weckx   Group: iGEM13_KU_Leuven   (2013-09-19)
Revision as of 23:05, 4 October 2013 by Dinox (Talk | contribs)

Medium constitutive expression of EBF synthase from Artemisia annua

For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 177
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal BamHI site found at 677
    Illegal XhoI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 249


Construct digestion

BBa_K1060009_gel_pic.jpg


System testing

Our pilot experiment with aphid indicated that our brick performed the desired function, for the video of aphids experiment, please go to [http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]. In this video you will find our first behavioural experiment with aphids. An EBF-producing bacterium plate (E. coli BL21(DE3) on LB with 5mM Mg2+ and Chloramphenicol is placed on the left, a control plate on the right, aphids are placed on the leaf in the middle. The first results indicate a positive trend, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.) For more information on the experimental setup and a discussion of the results go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphidexperiments here].

For more infromation see the experience page or go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphid%20experiments here].

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Parameters
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