Generator

Part:BBa_K1060003:Experience

Designed by: Ingmar Claes, Robbert Boudewijns, Laurens De Backer   Group: iGEM13_KU_Leuven   (2013-09-17)
Revision as of 00:20, 29 October 2013 by LMarcelis (Talk | contribs) (→‎System testing)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQa61d9bc224c4d6b2-partinfo-00000000-QINU UNIQa61d9bc224c4d6b2-partinfo-00000001-QINU

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Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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