Tag
cln2

Part:BBa_K105014

Designed by: Robin Sorg   Group: iGEM08_ESBS-Strasbourg   (2008-10-19)

cln2 PEST destabilization domain for rapid protein turnover

This BioBrick is the C-terminal domain of the yeast cln2 protein. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/degradation
Parameters
None