Coding

Part:BBa_K1039012

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-12)
Revision as of 02:08, 13 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PhiC31 integrase

PhiC31 integrase is a serine integrase that catalyzes site-specific recombination between PhiC31 attP (BBa_K103900) and attB (Bba_K1039036) sites to produce PhiC31 attR (Bba_K1039037) and attL (Bba_K1039038) sites. This recombination is unidirectional, i.e. attR and attL do not recombine in the presence of PhiC31 integrase alone. (1)

In concert with PhiC31 recombination directionality factor (RDF) (BBa_K1039013), PhiC31 integrase catalyzes site-specific recombination between PhiC31 attR and attL sites, to produce PhiC31 attP and attB sites. This recombination is also unideirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of PhiC31 integrase and RDF.

PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches BBa_K1039009 and BBa_K1039008 from PB state to RL state.

In concert with B PhiC31 RDF (BBa_K1039013), PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches BBa_K1039010 and BBa_K1039011 from RL state to PB state.

References: 1. Groth, Amy C., and Michele P. Calos. "Phage Integrases: Biology and Applications." Journal of Molecular Biology. 335.3 (2004): 667-678.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1665
    Illegal SapI.rc site found at 1356
    Illegal SapI.rc site found at 1414
    Illegal SapI.rc site found at 1519


[edit]
Categories
Parameters
None