Coding

Part:BBa_K1033115

Designed by: Sabri Jamal   Group: iGEM13_Uppsala   (2013-09-17)


Histidine Patch Thioredoxin

This is a mutant of E. coli's native thioredoxin. It has been modified to have the ability to chelate metal ions, notably nickel. This protein is useful as a fusion protein which can help to increase the solubility of difficult-to-express proteins and maximize yield of those proteins. Please note that this protein does not have a stop codon, to aid in its use as a fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None