Protein_Domain

Part:BBa_K103016

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-10-09)

OmpA_linker_omega_linker



Usage and Biology

  • It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit omega ( BBa_K103012)
  • Fusion partners are connected via Gly-Ser-Gly linker
  • It's base for creation of hunter fusions in 'hunter and prey' selection system
  • Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
  • Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard
 Ompalinkomega.jpg

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 802
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 596


[edit]
Categories
//cds/enzyme
//cds/membrane
//function/reporter
Parameters
None