Device

Part:BBa_K091182

Designed by: Robert Cool   Group: iGEM08_Davidson-Missouri_Western   (2008-08-05)

pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT

This construct uses two hybrid promoters. pLas/cI drives expression of the LuxI and Mnt genes. The promoter is designed to respond to activation by LasR when bound to P-AI-1 and to repression by lambda cI. pTet/Mnt drives expression of the LuxI and cI genes. It is designed for repression by the Tet repressor, which can be derepressed by aTc, and by the Mnt repressor. LuxI is a sythase that produces AI-1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 827
    Illegal BglII site found at 1356
    Illegal BglII site found at 2078
    Illegal XhoI site found at 1318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/cellsignalling
//function/regulation/transcriptional
Parameters
None