RBS
Part:BBa_J73036
Designed by: Eric Matthews Group: Miller Lab (2022-04-20)
Ethylene Forming Enzyme coding sequence
This sequence codes for the Ethylene Forming Enzyme (EFE) from the Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium. The EFE protein catalyzes the following reaction: AKG + 3 O2 + L-arginine → 2 ethylene + succinate + 7 CO2 + guanidine + P5C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 287
Illegal XhoI site found at 1012 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 265
Illegal AgeI site found at 1057 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 583
Illegal SapI site found at 888
Illegal SapI.rc site found at 387
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Categories
Parameters
None |