RBS

Part:BBa_J73036

Designed by: Eric Matthews   Group: Miller Lab   (2022-04-20)


Ethylene Forming Enzyme coding sequence

This sequence codes for the Ethylene Forming Enzyme (EFE) from the Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium. The EFE protein catalyzes the following reaction: AKG + 3 O2 + L-arginine → 2 ethylene + succinate + 7 CO2 + guanidine + P5C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal XhoI site found at 1012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 265
    Illegal AgeI site found at 1057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 583
    Illegal SapI site found at 888
    Illegal SapI.rc site found at 387


[edit]
Categories
Parameters
None